Col1a1^{tm1(tetO-Mir101a,-GFP)Plot}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-Mir101a,-GFP)Plot}
• Name: collagen, type I, alpha 1; targeted mutation 1, Lilian Plotkin
• MGI ID: MGI:7428859
• Synonyms: Col1a1^mir-101a
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-Mir101a,-GFP)Plot} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327373
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Col1a1^{tm1(tetO-Mir101a,-GFP)Plot} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mir101a (MGI:2676803)

• Mutation details: RMCE on KH2 cells replaced the FRT-flanked neo cassette with a cassette that contains the tetracycline promoter driving expression of the microRNA and and a GFP reporter gene. (J:327373)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:327373 Dudakovic A, et al., MicroRNA-101a enhances trabecular bone accrual in male mice. Sci Rep. 2022 Aug 3;12(1):13361
• All: 1 reference(s)