Del(14Rr299391-Rr308)2Fwa
Targeted Allele Detail

Summary

• Symbol: Del(14Rr299391-Rr308)2Fwa
• Name: deletion, chr 14, Frederick W Alt 2
• MGI ID: MGI:7386904
• Synonyms: EdeltadeltadeltaEdeltaR
• Gene: Del(14Rr299391-Rr308)2Fwa Location: unknown Genetic Position: Chr14, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82395
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Del(14Rr299391-Rr308)2Fwa involves 66 genes/genome features (Rr308, A430107P09Rik, Rr299391 ...) View all
• Mutation details: A 1085 bp region containing Tcrd enhancer Edelta was replaced with a loxP site flanked neomycin resistance gene cassette, which was subsequently removed via cre-mediated recombination. A 1 kb PvuII-BamHI genomic fragment containing Tcra enhancer Ealpha (Rr308) was replaced with a sequence containing a loxP site flanked neomycin resistance gene cassette and Tcrd enhancer Edelta (Rr299391). The neo cassette was subsequently deleted through cre-mediated recombination. Further cre-mediated recombination between the single loxP sites left in place of Edelta and upstream of the Edelta replacing Ealpha results in the deletion of Trdc, Trdv5, the entire Traj cluster and Trac. (J:82395)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(14Rr299391-Rr308)2Fwa Mutation: 0 strains or lines available

References

• Original: J:82395 Bassing CH, et al., T cell receptor (TCR) alpha/delta locus enhancer identity and position are critical for the assembly of TCR delta and alpha variable region genes. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2598-603
• All: 1 reference(s)