Igs2tm1(CAG-AU040320/mCherry,-cas9*)Janc
Targeted Allele Detail
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Symbol: |
Igs2tm1(CAG-AU040320/mCherry,-cas9*)Janc |
Name: |
intergenic site 2; targeted mutation 1, Jan Carette |
MGI ID: |
MGI:7384598 |
Synonyms: |
SELECTIV |
Gene: |
Igs2 Location: unknown Genetic Position: Chr11, Syntenic
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Alliance: |
Igs2tm1(CAG-AU040320/mCherry,-cas9*)Janc page
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Allele Type: |
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Targeted (Conditional ready, Inserted expressed sequence, Reporter) |
Mutation: |
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Insertion
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Igs2tm1(CAG-AU040320/mCherry,-cas9*)Janc expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Note |
mouse |
AU040320 (MGI:2140475) |
fused to mCherry |
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Mutation details: This allele was generated using PhiC31 integrase-mediated transgenesis. A plasmid containing from 5' to 3' a CAG promoter, a loxP-flanked stop sequence, mouse AU040320 (Aavr) fused to mCherry, an F2A self-cleaving peptide sequence, and a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [spCas9]) and PhiC31 integrase RNA was injected into the cytoplasm of C57BL/6 embryos carrying three tandem attP sites in the "safe harbor" Igs2 (H11) intergenic site, Igs2.
(J:341522)
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Original: |
J:341522 Zengel J, et al., Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression. Nat Methods. 2023 Jul;20(7):1070-1081 |
All: |
1 reference(s) |
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