Igs2^{tm1(CAG-AU040320/mCherry,-cas9*)Janc}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(CAG-AU040320/mCherry,-cas9*)Janc}
• Name: intergenic site 2; targeted mutation 1, Jan Carette
• MGI ID: MGI:7384598
• Synonyms: SELECTIV
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(CAG-AU040320/mCherry,-cas9*)Janc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341522
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: STOCK Igs2^tm3.1Luo/Luo

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Igs2^{tm1(CAG-AU040320/mCherry,-cas9*)Janc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	AU040320 (MGI:2140475)	fused to mCherry

• Mutation details: This allele was generated using PhiC31 integrase-mediated transgenesis. A plasmid containing from 5' to 3' a CAG promoter, a loxP-flanked stop sequence, mouse AU040320 (Aavr) fused to mCherry, an F2A self-cleaving peptide sequence, and a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [spCas9]) and PhiC31 integrase RNA was injected into the cytoplasm of C57BL/6 embryos carrying three tandem attP sites in the "safe harbor" Igs2 (H11) intergenic site, Igs2. (J:341522)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying the Igs2^tm3.1Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 ES cells on a mixed background with 129S1/SvJ, 129X1/SvJ, C57BL/6J, DBA/2J, and FVB/N.

References

• Original: J:341522 Zengel J, et al., Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression. Nat Methods. 2023 Jul;20(7):1070-1081
• All: 2 reference(s)