Laptm5^em#Zhaw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Laptm5^em#Zhaw
• Name: lysosomal-associated protein transmembrane 5; endonuclease-mediated mutation, Wenyuan Zhao
• MGI ID: MGI:7335157
• Gene: Laptm5 Location: Chr4:130640627-130663459 bp, + strand Genetic Position: Chr4, 64.0 cM
• Alliance: Laptm5^em#Zhaw page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination using the single guide RNA CCAGGGCTATGGTGGCGAC targeting the upstream exon created a null allele. Western blot analysis confirmed the absence of protein expression in brains from homozygous mice. (J:327929)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Laptm5 Mutation: 35 strains or lines available

References

• Original: J:327929 Zhang Z, et al., Lysosomal-associated transmembrane protein 5 deficiency exacerbates cerebral ischemia/reperfusion injury. Front Mol Neurosci. 2022;15:971361
• All: 1 reference(s)