Pkm^tm2.1Mgvh
Targeted Allele Detail

Summary

• Symbol: Pkm^tm2.1Mgvh
• Name: pyruvate kinase, muscle; targeted mutation 2.1, Matthew G Vander Heiden
• MGI ID: MGI:7331322
• Synonyms: Pkm1^fl
• Gene: Pkm Location: Chr9:59563859-59586655 bp, + strand Genetic Position: Chr9, 32.03 cM
• Alliance: Pkm^tm2.1Mgvh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326292
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Modified isoform(s))
• Mutation: Insertion
• Mutation details: A targeting vector was designed to contain a FRT-flanked neomycin cassette upstream of exon 9 and loxP sites that flank exon 9, which is alternatively spliced only in the Pkm1 isoform. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 9 deleted in the cre-expressing tissues: pyruvate kinase M1 isoform expression is eliminated, while pyruvate kinase M2 isoform expression is unchanged. Flp-mediated recombination removed the neo cassette. (J:326292)

Tumor Data

List all tumor models in MMHCdb carrying Pkm^tm2.1Mgvh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pkm Mutation: 80 strains or lines available

References

• Original: J:326292 Davidson SM, et al., Pyruvate Kinase M1 Suppresses Development and Progression of Prostate Adenocarcinoma. Cancer Res. 2022 Jul 5;82(13):2403-2416
• All: 6 reference(s)