Orc4^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Orc4^{tm1c(EUCOMM)Wtsi}
• Name: origin recognition complex, subunit 4; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7314200
• Gene: Orc4 Location: Chr2:48792836-48840287 bp, - strand Genetic Position: Chr2, 28.42 cM
• Alliance: Orc4^{tm1c(EUCOMM)Wtsi} page
• IMPC: Orc4 gene page

Mutation origin

• Mutant Cell Line: EPD0087_3_G06
• Germline Transmission: Earliest citation of germline transmission: J:326522
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 48819432 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 48820222. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:326522)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Orc4 Mutation: 54 strains or lines available

References

• Original: J:326522 Nguyen H, et al., Deletion of Orc4 during oogenesis severely reduces polar body extrusion and blocks zygotic DNA replication. Biol Reprod. 2022;106(4):730-740
• All: 1 reference(s)