Antxr1^em1Bstc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Antxr1^em1Bstc
• Name: anthrax toxin receptor 1; endonuclease-mediated mutation 1, Brad St. Croix
• MGI ID: MGI:7310435
• Synonyms: TEM8^E150V
• Gene: Antxr1 Location: Chr6:87110835-87312757 bp, - strand Genetic Position: Chr6, 37.94 cM
• Alliance: Antxr1^em1Bstc page

Mutation origin

• Strain of Origin: C57BL/6Ncr x B6D2F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Two guide RNAs (5'-TCATCATCGCGTTGACGGAT-3', PAM=GGG; 5'- GACGGATGGGGAGCTGCACG-3', PAM=AGG) and a single-stranded DNA containing the E150V mutation is used to create a GAG to GTA missense mutation. A silent mutation was also added, creating a ScaI restriction site (AGTACT). This mutation hinders the binding of ANTRX1 to collagen. (J:331389)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Antxr1 Mutation: 29 strains or lines available

References

• Original: J:331389 Hsu KS, et al., Cancer cell survival depends on collagen uptake into tumor-associated stroma. Nat Commun. 2022 Nov 18;13(1):7078
• All: 1 reference(s)