Wiz^em1Ccpcz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Wiz^em1Ccpcz
• Name: widely-interspaced zinc finger motifs; endonuclease-mediated mutation 1, Institute of Molecular Genetics
• MGI ID: MGI:7310064
• Gene: Wiz Location: Chr17:32573029-32608413 bp, - strand Genetic Position: Chr17, 17.52 cM, cytoband B2
• Alliance: Wiz^em1Ccpcz page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination using guide RNAs targeting the zinc-finger domain in exon 4 created 32 nucleotide deletion and mismatch of 4 nucleotides causing a frame-shift mutation and premature stop codon. Western blot analysis confirmed the absence of protein expression in homozygous embryos. (J:308697)

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wiz Mutation: 60 strains or lines available

References

• Original: J:308697 Bukova I, et al., Loss of Wiz Function Affects Methylation Pattern in Palate Development and Leads to Cleft Palate. Front Cell Dev Biol. 2021;9:620692
• All: 1 reference(s)