Igs2^{em63(tetO-TadA*V106W/cas9,CAG-rtTA)Gpt}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs2^{em63(tetO-TadA*V106W/cas9,CAG-rtTA)Gpt}
• Name: intergenic site 2; endonuclease-mediated mutation 63, GemPharmatech Co., Ltd
• MGI ID: MGI:7300676
• Synonyms: Igs2^{em1Cin(pTRE3G-ABE8e(V106W)-rTTA-CAG)}
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{em63(tetO-TadA*V106W/cas9,CAG-rtTA)Gpt} page

Mutation origin

• Strain of Origin: C57BL/6JGpt

Mutation description

• Allele Type: Endonuclease-mediated (Endonuclease, Inducible, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted the tetracycline response element driving inducible expression of the E. coli tRNA adenosine deaminase with the mutation encoding the amino acid substitution of valine with tryptophan at position 106 (V106W) fused with cas9 endonuclease. The insertion also carries a cassette composed of a CAG promoter and reverse tetracycline transactivator. (J:293217)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

References

• Original: J:293217 GemPharmatech, GemPharmatech Website. 2020
• All: 1 reference(s)