Sftpc^{tm1.1(Nicre)Thbr}
Targeted Allele Detail

Summary

• Symbol: Sftpc^{tm1.1(Nicre)Thbr}
• Name: surfactant associated protein C; targeted mutation 1.1, Thomas Braun
• MGI ID: MGI:7263083
• Synonyms: SPC^{-2A-YFP-2A-iCre-N}
• Gene: Sftpc Location: Chr14:70758389-70761521 bp, - strand Genetic Position: Chr14, 36.32 cM
• Alliance: Sftpc^{tm1.1(Nicre)Thbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323339
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Epitope tag, Recombinase, Reporter)
• Mutation: Insertion
• Sftpc^{tm1.1(Nicre)Thbr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sftpc (MGI:109517)

• Mutation details: Mice were generated by inserting a 2A sequence, YFP, 2A sequence, and the N-terminal fragment of improved cre (iCre) recombinase (aa 1-104) fused to the N terminal 102 amino acids of the DnaE split intein sequence from Nostoc punctiforme (Npu), FLAG tag, and Frt-flanked neomycin resistance cassette immediately in front of the stop codon of Sftpc coding sequence by homologous recombination. The Frt-flanked neomycin resistance cassette was removed by crossing with hACTB-Flpe deleter mice. (J:323339)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sftpc (mouse) Summary of all recombinase alleles driven by Sftpc. Comparative matrix view of recombinase activities Alliance mouse Sftpc gene page

Expression

In Mice Carrying this Mutation:

2 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sftpc Mutation: 23 strains or lines available

References

• Original: J:323339 Salwig I, et al., Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia in vivo. EMBO J. 2019 Jun 17;38(12):e102099
• All: 1 reference(s)