Fcgrt^{em1(FCGRT)Synbl}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fcgrt^{em1(FCGRT)Synbl}
• Name: Fc fragment of IgG receptor and transporter; endonuclease-mediated mutation 1, Synbal
• MGI ID: MGI:7261380
• Gene: Fcgrt Location: Chr7:44742417-44753246 bp, - strand Genetic Position: Chr7, 29.12 cM
• Alliance: Fcgrt^{em1(FCGRT)Synbl} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Fcgrt^{em1(FCGRT)Synbl} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FCGRT (2217)

  Fcgrt

  (MGI:103017)

• Mutation details: Cas9 and gRNA Fcgrt-g91T (protospacer sequence 5- GGGTCACCCTGTCGGAATG-3) are used to introduce the following sequence at the start codon in exon 2: Kozak sequence and human FCGRT cDNA cassette inserted at the cleavage site, Sv40 intron, a 214 bp native hFCGRT 3UTR (3UTR), a 152 bp native human sequence 3 of the mapped 3UTR, and a mouse U6 promoter-driven guide RNA scaffold with 2xPolIII terminator (all in reverse orientation immediately 3 of the hFCGRT cassette), a splice-trap element with splice acceptor and SV40 polyadenylation sequence followed by 3-frame ATG short open reading frames. This active genetics cassette is designed to trap and truncate any mouse Fcgrt isoforms that may splice past the hFCGRT cassette. A Flp Recombinase Target (FRT) site is inserted to allow Flp-mediated collapse of targeted tandem integration events (if needed). (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Fcgrt Mutation: 60 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 2 reference(s)