Mettl5^em1.1Snac
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mettl5^em1.1Snac
• Name: methyltransferase 5, N6-adenosine; endonuclease-mediated mutation 1.1, Sigrid Nachtergaele
• MGI ID: MGI:7260126
• Gene: Mettl5 Location: Chr2:69701542-69715959 bp, - strand Genetic Position: Chr2, 40.95 cM, cytoband C3
• Alliance: Mettl5^em1.1Snac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:322368
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6 x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: A Mettl5 conditional knockout line was made in which exon 2 was floxed, such that the presence of Cre would induce exon 2 deletion by creating a frame-shift mutation. An FRT-flanked neomycin cassette included in the construct was excised. The construct was electroporated with gRNA/Cas9 protein into ES cells that are F1 hybrid of C57bl/6 x 129S6. (J:322368)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mettl5 Mutation: 41 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:322368 Sepich-Poore C, et al., The METTL5-TRMT112 N(6)-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation. J Biol Chem. 2022 Jan 14;298(3):101590
• All: 1 reference(s)