Dnmt3b^em1Rop
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnmt3b^em1Rop
• Name: DNA methyltransferase 3B; endonuclease-mediated mutation 1, Rene Opavsky
• MGI ID: MGI:6833877
• Synonyms: Dnmt3b^CI, Dnmt3b^P656V/C657D
• Gene: Dnmt3b Location: Chr2:153491370-153529650 bp, + strand Genetic Position: Chr2, 75.79 cM, cytoband A2-A3
• Alliance: Dnmt3b^em1Rop page

Mutation origin

• Strain of Origin: FVB/N

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology generated a CCATGC to GTCGAC change resulting in a proline to valine substitution at amino acid 656 (P656V) and a cysteine to aspartate substitution at position 657 (C657D) in exon 19. These substitutions are predicted to disable the first two steps (nucleophilic addition and methyl transfer) of the three-step process required for catalytic activity and thus result in a catalytically inactive protein. (J:279449)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3b Mutation: 53 strains or lines available

References

• Original: J:279449 Nowialis P, et al., Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions. Nat Commun. 2019 Sep 26;10(1):4374
• All: 3 reference(s)