Tg(tetO-Neurl1a*)NFC147Kndl
Transgene Detail

Summary

• Symbol: Tg(tetO-Neurl1a*)NFC147Kndl
• Name: transgene insertion NFC147, Eric R Kandel
• MGI ID: MGI:6831636
• Synonyms: tetO-Neurl1DN-Flag
• Transgene: Tg(tetO-Neurl1a*)NFC147Kndl Location: unknown
• Alliance: Tg(tetO-Neurl1a*)NFC147Kndl page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-Neurl1a*)NFC147Kndl expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Neurl1a (MGI:1334263)	amino acids 1-520 fused to a FLAG epitope

• Mutation details: An open reading frame corresponding to amino acids 1-520 of the mouse Neurl1a gene (Genbank: BC099702) is fused to a Flag tag epitope and subsequently cloned into tetO plasmid pMM400. An SV40 polyadenylation (polyA) site in the plasmid was replaced with a human growth hormone (HGH) polyA site. This is a dominant-negative form of Neurl1a that lacks its RING Zn finger, the domain that possesses the ubiquitin ligase activity. (J:178916)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:178916 Pavlopoulos E, et al., Neuralized1 Activates CPEB3: A Function for Nonproteolytic Ubiquitin in Synaptic Plasticity and Memory Storage. Cell. 2011 Dec 9;147(6):1369-83
• All: 1 reference(s)