Igs2^{tm1(Smarcd3-lacZ)Bbr}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(Smarcd3-lacZ)Bbr}
• Name: intergenic site 2; targeted mutation 1, Benoit G Bruneau
• MGI ID: MGI:6790571
• Synonyms: F6nLacZ, Smarcd3-F6nLacZ
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(Smarcd3-lacZ)Bbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232545
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: STOCK Igs2^tm3.1Luo/Luo

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: Site-specific PhiC31 integrase-mediated transgenesis is used to insert H19 insulator flanked fragment of the Smarcd3-F6 fragment, including approximately 2.5 kb of the 5 end of the Smarcd3-F1 enhancer and Hsp68 minimal promoter followed by nLacZ coding sequence and EF1 poly siganal into the intergenic site 2 locus (Hipp11). This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice. (J:232545)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 85 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying the Igs2^tm3.1Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 ES cells on a mixed background with 129S1/SvJ, 129X1/SvJ, C57BL/6J, DBA/2J, and FVB/N.

References

• Original: J:232545 Devine WP, et al., Early patterning and specification of cardiac progenitors in gastrulating mesoderm. Elife. 2014;3
• All: 1 reference(s)