Gt(ROSA)26Sor^{tm9(CAG-mEYFP*,-tdTomato*,-Notch1*,-mKate2*,-Rbpj*)Ben}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm9(CAG-mEYFP*,-tdTomato*,-Notch1*,-mKate2*,-Rbpj*)Ben}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 9, Rui Benedito
• MGI ID: MGI:6761546
• Synonyms: Gt(Rosa)26Sor^{tm1(iMb-Notch-Mosaic)}, iMb-Notch-Mosaic
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm9(CAG-mEYFP*,-tdTomato*,-Notch1*,-mKate2*,-Rbpj*)Ben} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252386
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm9(CAG-mEYFP*,-tdTomato*,-Notch1*,-mKate2*,-Rbpj*)Ben} expresses 2 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Notch1 (MGI:97363)	sequence coding for the intra-cellular and PEST domains is downstream in-frame with T2A sequence which is downstream in-frame with tdTomato gene
  mouse	Rbpj (MGI:96522)	sequence coding for mutated mouse peptide with p.R218H substitution is downstream in-frame with T2A sequence which is downstream in-frame with mKate2 gene

• Mutation details: This allele has three membrane-targeted fluorescent protein sequences - each uniquely flanked with pairs of incompatible lox sites (in the same direction) inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus with a CAG hybrid promoter/enhancer. The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxN site, a lox2272 site, a loxP site, a neomycin resistance gene cassette, bovine GH polyA signal sequence, a second loxN site, the MbYFP gene (monomeric enhanced yellow fluorescent protein [mYFP^A206K] with N-terminal membrane-targeting tag), in-frame Thosea asigna virus 2A cleavage site (T2A; mediates ribosomal skipping), in-frame bovine papillomavirus-1 AU1 tag sequence, in-frame T2A sequence, bovine GH polyA signal sequence, a second lox2272 site, the MbTomato gene (non-oligomerizing tandem-dimer DsRed fluorescent protein variant tdTomato with N-terminal membrane-targeting tag sequence), in-frame T2A sequence, in-frame AU1 tag sequence, in-frame T2A sequence, in-frame mouse Notch1 intracellular domain (NICD) and PEST domain sequence (NICD-PEST), bovine GH polyA signal sequence, a second loxP site, the MbKate2 gene (monomeric far-red fluorescent protein mKate2 with N-terminal membrane-targeting tag sequence), in-frame T2A sequence, in-frame AU1 tag sequence, in-frame T2A sequence, in-frame sequence for a dominant-negative form of mouse Rbpj (DN-Rbpj, with a p.R218H mutation), and bovine GH polyA signal sequence. (J:252386)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:252386 Pontes-Quero S, et al., Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis. Cell. 2017 Aug 10;170(4):800-814.e18
• All: 1 reference(s)