Crhr2^{tm1.1(cre)Lbrl}
Targeted Allele Detail

Summary

• Symbol: Crhr2^{tm1.1(cre)Lbrl}
• Name: corticotropin releasing hormone receptor 2; targeted mutation 1.1, Stephen Liberles
• MGI ID: MGI:6726589
• Synonyms: Crhr2-ires-Cre
• Gene: Crhr2 Location: Chr6:55067034-55110001 bp, - strand Genetic Position: Chr6, 27.33 cM
• Alliance: Crhr2^{tm1.1(cre)Lbrl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:298576
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Crhr2^{tm1.1(cre)Lbrl} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Crhr2 (MGI:894312)

• Mutation details: A targeting construct was designed to insert an internal ribosomal entry site (IRES) and a Cre Recombinase with a polyadenylation signal, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of the stop codon of the corticotropin releasing hormone receptor 2 (Crhr2) gene. The neomycin resistance gene was subsequently removed by crossing with Act-Flpe mice. (J:298576)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Crhr2 (mouse) Summary of all recombinase alleles driven by Crhr2. Comparative matrix view of recombinase activities Alliance mouse Crhr2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Crhr2 Mutation: 34 strains or lines available

References

• Original: J:298576 Prescott SL, et al., An Airway Protection Program Revealed by Sweeping Genetic Control of Vagal Afferents. Cell. 2020 Apr 30;181(3):574-589.e14
• All: 2 reference(s)