Mest^tm1.2Rkz
Targeted Allele Detail

Summary

• Symbol: Mest^tm1.2Rkz
• Name: mesoderm specific transcript; targeted mutation 1.2, Robert Koza
• MGI ID: MGI:6724093
• Synonyms: Mest^gKO
• Gene: Mest Location: Chr6:30723546-30748464 bp, + strand Genetic Position: Chr6, 12.53 cM, cytoband B1
• Alliance: Mest^tm1.2Rkz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245106
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The targeting vector contains a loxP-flanked neomycin cassette introduced to intron 2 and a loxP site in intron 3. Cre-mediated recombination removed exon 3 and the neo cassette. Deletion of exon 3 causes a frameshift mutation and the generation of a stop codon in exon 4. qRT-PCR analysis in adipose tissue shows complete loss of mRNA. (J:245106)

Expression

In Mice Carrying this Mutation:

7 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mest Mutation: 27 strains or lines available

References

• Original: J:245106 Anunciado-Koza RP, et al., Diet-induced adipose tissue expansion is mitigated in mice with a targeted inactivation of mesoderm specific transcript (Mest). PLoS One. 2017;12(6):e0179879
• All: 2 reference(s)