Fbxo10^em2Ccg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fbxo10^em2Ccg
• Name: F-box protein 10; endonuclease-mediated mutation 2, Christopher C Goodnow
• MGI ID: MGI:6721015
• Synonyms: Fbxo10^frameshift, Fbxo10^fs, Fbxo10^KO
• Gene: Fbxo10 Location: Chr4:45034248-45084604 bp, - strand Genetic Position: Chr4, 23.68 cM
• Alliance: Fbxo10^em2Ccg page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 4 nucleotide deletion (GCCG) in codons 55 and 56 within exon 2, changing the cysteine 55 codon to serine and creating a reading frame shift and premature stop codon after 55 codons (c.del285_288; p.C55Sfs*55). The frame shift deletion does not create a new splice donor site and there is no evidence of alternative splice forms that skip exon 2 and thus is expected to create a null allele. However, antibodies to check for protein expression are not available. (J:307413)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fbxo10 Mutation: 49 strains or lines available

References

• Original: J:307413 Masle-Farquhar E, et al., Loss-of-function of Fbxo10, encoding a post-translational regulator of BCL2 in lymphomas, has no discernible effect on BCL2 or B lymphocyte accumulation in mice. PLoS One. 2021;16(4):e0237830
• All: 1 reference(s)