Arhgef7^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Arhgef7^{tm1d(EUCOMM)Wtsi}
• Name: Rho guanine nucleotide exchange factor; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6501603
• Gene: Arhgef7 Location: Chr8:11778053-11885219 bp, + strand Genetic Position: Chr8, 5.73 cM
• Alliance: Arhgef7^{tm1d(EUCOMM)Wtsi} page
• IMPC: Arhgef7 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217373
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 11841010 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 11841787. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:217373)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arhgef7 Mutation: 53 strains or lines available

References

• Original: J:217373 Omelchenko T, et al., beta-Pix directs collective migration of anterior visceral endoderm cells in the early mouse embryo. Genes Dev. 2014 Dec 15;28(24):2764-77
• All: 2 reference(s)