Col1a1^{tm2(tetO-EWSR1/ATF1,-mCherry)Yasu}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2(tetO-EWSR1/ATF1,-mCherry)Yasu}
• Name: collagen, type I, alpha 1; targeted mutation 2, Yasuhiro Yamada
• MGI ID: MGI:6470985
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2(tetO-EWSR1/ATF1,-mCherry)Yasu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279289
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: RMCE knocked in the construct containing a tetracycline responsive element driving inducible expression of the human fusion sequence from CCS cell line KAS with an IRES sequence and an mCherry reporter gene into the 3' UTR. (J:279289)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 161 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae} (J:159351).

References

• Original: J:279289 Komura S, et al., Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas. Nat Commun. 2019 Sep 5;10(1):3999
• All: 2 reference(s)