Dynlrb1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dynlrb1^{tm1c(EUCOMM)Wtsi}
• Name: dynein light chain roadblock-type 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6466548
• Synonyms: Dynlrb^tm1c
• Gene: Dynlrb1 Location: Chr2:155078453-155092197 bp, + strand Genetic Position: Chr2, 77.07 cM
• Alliance: Dynlrb1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Dynlrb1 gene page

Mutation origin

• Mutant Cell Line: EPD0373_7_C01
• Germline Transmission: Earliest citation of germline transmission: J:292124
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 155089269 of Chromosome 2 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 155090135. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving exon 3 flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:292124)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dynlrb1 Mutation: 12 strains or lines available

References

• Original: J:292124 Terenzio M, et al., DYNLRB1 is essential for dynein mediated transport and neuronal survival. Neurobiol Dis. 2020 Jul;140:104816
• All: 1 reference(s)