Fimp1^em2Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fimp1^em2Osb
• Name: fertilization influencing membrane protein 1; endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6460467
• Gene: Fimp1 Location: Chr7:126429640-126430712 bp, - strand Genetic Position: Chr7, 69.25 cM
• Alliance: Fimp1^em2Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination created a 246 bp deletion around exon 3, including 88bp from exon 3 and 12bp from exon 4 resulting in an in-frame mutation and premature stop codon at the 85th amino acid. This is predicted to disrupt only the transmembrane isoform. RT-PCR analysis confirmed the absence of the transmembrane form and presence of the secreted form. (J:287619)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Fimp1 Mutation: 16 strains or lines available

References

• Original: J:287619 Fujihara Y, et al., Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice. Proc Natl Acad Sci U S A. 2020 Apr 28;117(17):9393-9400
• All: 1 reference(s)