Rr427^tm1Afrdm
Targeted Allele Detail

Summary

• Symbol: Rr427^tm1Afrdm
• Name: regulatory region 427; targeted mutation 1, Alan Friedman
• MGI ID: MGI:6455194
• Synonyms: Cebpa^tm1Afrdm, Enh^f
• Gene: Rr427 Location: unknown Genetic Position: Chr7, Syntenic
• Alliance: Rr427^tm1Afrdm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294260
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Modified regulatory region)
• Mutation: Insertion
• Mutation details: The targeting vector is designed to insert a loxP site upstream of the 440 bp enhancer sequence that is centered at +37.5 kb downstream of the Cebpa transcriptional start site. An FRT-flanked neomycin resistance (neo) cassette, followed by a second loxP site was placed downstream of the +37 kb enhancer. (J:294260)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr427 Mutation: 0 strains or lines available

Notes

BL-1 ES cell line - C57BL/6

References

• Original: J:294260 Guo H, et al., In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis. PLoS One. 2016;11(3):e0150809
• All: 3 reference(s)