Dtl^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dtl^{tm1c(EUCOMM)Wtsi}
• Name: denticleless E3 ubiquitin protein ligase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6437412
• Synonyms: Dcaf2^fl
• Gene: Dtl Location: Chr1:191269468-191307656 bp, - strand Genetic Position: Chr1, 96.44 cM, cytoband H6
• Alliance: Dtl^{tm1c(EUCOMM)Wtsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245637
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 191293225 of Chromosome 1 upstream of the critical exons 6-7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 191295597. The critical exons 6-7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele, to remove the lacZ sequence and selection cassette, leaving loxP sites flanking the critical exons 6-7. (J:245637)

Expression

In Mice Carrying this Mutation:

39 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dtl Mutation: 47 strains or lines available

References

• Original: J:245637 Xu YW, et al., Maternal DCAF2 is crucial for maintenance of genome stability during the first cell cycle in mice. J Cell Sci. 2017 Oct 01;130(19):3297-3307
• All: 4 reference(s)