Tg(S1pr2-cre/ERT2)#Kuro
Transgene Detail

Summary

• Symbol: Tg(S1pr2-cre/ERT2)#Kuro
• Name: transgene insertion, Tomohiro Kurosaki
• MGI ID: MGI:6435090
• Synonyms: S1pr2-ERT2cre
• Transgene: Tg(S1pr2-cre/ERT2)#Kuro Location: unknown
• Alliance: Tg(S1pr2-cre/ERT2)#Kuro page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(S1pr2-cre/ERT2)#Kuro expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	S1pr2 (MGI:99569)

• Mutation details: An ERT2cre-encoding gene fused with a poly(A) signal and a neomycin-kanamycin resistance gene flanked with two FRT sites were inserted into the exon 2 of the S1pr2 gene, which encodes S1P2, on a BAC clone (RPCI23.C-168D6). Correctly targeted clones were injected into pronuclei of C57BL/6 zygotes to generate transgenic funder lines. The founder mice were identified by PCR and were further crossed with CAG-Flp transgenic mice to remove the neomycin-kanamycin resistance gene cassette. Line numbers were not indicated. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:260241)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	S1pr2 (mouse) Summary of all recombinase alleles driven by S1pr2. Alliance mouse S1pr2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:260241 Shinnakasu R, et al., Regulated selection of germinal-center cells into the memory B cell compartment. Nat Immunol. 2016 Jul;17(7):861-9
• All: 31 reference(s)