Stk39^tm1Pawe
Targeted Allele Detail

Summary

• Symbol: Stk39^tm1Pawe
• Name: serine/threonine kinase 39; targeted mutation 1, Paul A Welling
• MGI ID: MGI:6423627
• Synonyms: floxed CA-SPAK
• Gene: Stk39 Location: Chr2:68040789-68302381 bp, - strand Genetic Position: Chr2, 39.53 cM, cytoband C3
• Alliance: Stk39^tm1Pawe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287773
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP flanked PGK-neomycin selection cassette and an HA-tagged Stk39 cDNA containing two mutations, a threonine to glutamate change at residue 243 (T243E) and a serine to aspartate change at 383 (S383D) and an in-frame stop codon were inserted after the start codon of Stk39. These two mutations generate a constitutively active kinase. In the absence of cre-recombinase, the cassette renders the native gene inactive and generates a null allele. (J:287773)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stk39 Mutation: 42 strains or lines available

References

• Original: J:287773 Grimm PR, et al., Constitutively Active SPAK Causes Hyperkalemia by Activating NCC and Remodeling Distal Tubules. J Am Soc Nephrol. 2017 Sep;28(9):2597-2606
• All: 3 reference(s)