Gt(ROSA)26Sor^{tm1(CAG-mCherry/ATP2B2)Khakh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-mCherry/ATP2B2)Khakh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Baljit Khakh
• MGI ID: MGI:6416540
• Synonyms: CalEx^flox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-mCherry/ATP2B2)Khakh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:316361
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-mCherry/ATP2B2)Khakh} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ATP1B2 (482)

  Atp1b2

  (MGI:88109)

  variant w/b fused to mCherry

• Mutation details: The targeting vector contains (from 5' to 3') a ubiquitously expressed CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a single FRT site, loxP-flanked stop cassette (containing 3x SV40 polyadenylation [pA] sites), a red fluorescent protein (mCherry) tagged human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2) splice variant w/b sequence, an SV40pA site followed by a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and a neomycin resistance (neo) cassette followed by another SV40pA site. (J:316361)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:316361 Yu X, et al., Local and CNS-Wide Astrocyte Intracellular Calcium Signaling Attenuation In Vivo with CalEx(flox) Mice. J Neurosci. 2021 May 26;41(21):4556-4574
• All: 4 reference(s)