Mapt^{tm1.1(MAPT)Tcs}
Targeted Allele Detail

Summary

• Symbol: Mapt^{tm1.1(MAPT)Tcs}
• Name: microtubule-associated protein tau; targeted mutation 1.1, Takaomi C Saido
• MGI ID: MGI:6414668
• Synonyms: hTau-KI
• Gene: Mapt Location: Chr11:104122216-104222916 bp, + strand Genetic Position: Chr11, 67.79 cM, cytoband E1
• Alliance: Mapt^{tm1.1(MAPT)Tcs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278451
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The human MAPT sequence from exon 1 to exon 14 (NCBI Reference Sequence: NG_007398 with H2 haplotype) and a loxP/FRT flanked neomycin resistance gene replaced the entire genomic sequence (from exon 1 to exon 14) of murine Mapt via homologous recombination. Cre-mediated recombination removed the neomycin gene. Western blot analysis confirmed the expression of all human tau isoforms. (J:278451)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Mapt Mutation: 428 strains or lines available

References

• Original: J:278451 Hashimoto S, et al., Tau binding protein CAPON induces tau aggregation and neurodegeneration. Nat Commun. 2019 Jun 3;10(1):2394
• All: 3 reference(s)