Srebf2^{em1(KOMP)Wtsi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Srebf2^{em1(KOMP)Wtsi}
• Name: sterol regulatory element binding factor 2; endonuclease-mediated mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6406765
• Synonyms: Srebf2^{tm1a(KOMP)Wtsi}
• Gene: Srebf2 Location: Chr15:82031455-82089580 bp, + strand Genetic Position: Chr15, 38.49 cM
• Alliance: Srebf2^{em1(KOMP)Wtsi} page
• IMPC: Srebf2 gene page

Mutation origin

• Mutant Cell Line: BEPD0096_A12
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted upstream of the critical exon 3. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Srebf2 Mutation: 79 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)