Pde8b^{Tg(Alb-NAT2)1Kss}
Transgenic Allele Detail

Summary

• Symbol: Pde8b^{Tg(Alb-NAT2)1Kss}
• Name: phosphodiesterase 8B; transgene insertion 1, Kim Sugamori
• MGI ID: MGI:6391177
• Synonyms: hNAT2^tg
• Gene: Pde8b Location: Chr13:95160962-95386844 bp, - strand Genetic Position: Chr13, 49.22 cM
• Alliance: Pde8b^{Tg(Alb-NAT2)1Kss} page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Pde8b^{Tg(Alb-NAT2)1Kss} expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  NAT2 (10)

  Nat1	(MGI:97279)
  Nat2	(MGI:109201)
  Nat3	(MGI:102537)

• Mutation details: The human NAT2 reference wild-type (NAT2*4) gene is under the control of an approximate 2.3-kb mouse albumin enhancer/promoter. The transgene inserted in intron 3 of the mouse Pde8b gene. A recombination event during insertion caused transposition of most of the albumin enhancer region to the 3 end of the transgene construct, but does not markedly impair expression of the human transcript in the liver. Real-time qPCR indicates that transgenic mice have an approximate 40-55% decrease in Pde8b expression in the brain. Real-time qPCR also indicates that the human transcript is detected in the liver and is 2-fold higher in male liver than in female liver and Western blot analysis confirmed expression, with higher level of protein in the male liver than female liver. This line contains a single copy of the transgene. (J:284698)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pde8b Mutation: 57 strains or lines available

References

• Original: J:284698 Sugamori KS, et al., Liver-selective expression of human arylamine N-acetyltransferase NAT2 in transgenic mice. Drug Metab Dispos. 2011 May;39(5):882-90
• All: 1 reference(s)