Nr3c1^tm2.1Jaci
Targeted Allele Detail

Summary

• Symbol: Nr3c1^tm2.1Jaci
• Name: nuclear receptor subfamily 3, group C, member 1; targeted mutation 2.1, John A Cidlowski
• MGI ID: MGI:6385531
• Synonyms: Nr3c1^C3
• Gene: Nr3c1 Location: Chr18:39543598-39652474 bp, - strand Genetic Position: Chr18, 21.09 cM
• Alliance: Nr3c1^tm2.1Jaci page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282764
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting exchange vector was designed to contain exon 2 in which the ATG start codons for the translational isoforms GR-A, GR-B, GR-C1, GR-C2, GR-D1, GR-D2, and GR-D3 were mutated to ATC (isoleucine) except the start codon for GR-C3, which was left intact. In addition, a lox66 site followed by a hygromycin resistance gene cassette flanked by FRT sites was placed into intron 1 and a lox2272 site downstream of the mutated exon 2. This exchange vector and cre recombinase were transfected into embryonic stem cells in which exon 2 was replaced by a neomycin resistance gene cassette flanked by an upstream lox71 site and downstream lox2272 site. Cre-mediated recombination results in exchange of the neo cassette with the mutated exon 2. Flp-mediated recombination removed the hygro cassette. Mice exclusively express the GR-C3 isoform, which lacks the first 97 aa of the N-terminal domain. (J:282764)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nr3c1 Mutation: 36 strains or lines available

Notes

An embryonic stem (ES) cell line in which exon 2 of Nr3c1 was replaced by a neomycin cassette flanked by an upstream lox71 site and downstream lox2272 site was generated to create this allele.

References

• Original: J:282764 Oakley RH, et al., Glucocorticoid receptor isoform-specific regulation of development, circadian rhythm, and inflammation in mice. FASEB J. 2018 Oct;32(10):5258-5271
• All: 1 reference(s)