Scfd1^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Scfd1^em1(IMPC)Tcp
• Name: Sec1 family domain containing 1; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:6383384
• Gene: Scfd1 Location: Chr12:51424296-51496887 bp, + strand Genetic Position: Chr12, 22.11 cM, cytoband C1
• Alliance: Scfd1^em1(IMPC)Tcp page
• IMPC: Scfd1 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR1478 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of GCTTAGTGACTGATCGCAGT targeting the 5' side and CAAAACAACTACGGAGTTAG targeting the 3' side of a critical region. This resulted in a 1621-bp deletion Chr12:51388915-51390535 (GRCm38). (J:265051)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Scfd1 Mutation: 34 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 2 reference(s)