Pde5a^em1Laj
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pde5a^em1Laj
• Name: phosphodiesterase 5A, cGMP-specific; endonuclease-mediated mutation 1, Laurinda A Jaffe
• MGI ID: MGI:6368794
• Synonyms: Pde5^S92A
• Gene: Pde5a Location: Chr3:122522822-122653023 bp, + strand Genetic Position: Chr3, 53.69 cM
• Alliance: Pde5a^em1Laj page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: CRISPR/Cas9 technology generated a T to G point mutation at position 273 (T273G) that changes serine 92 to alanine. In addition, two silent point mutations (C263T and A269G) were generated to disrupt cutting by Cas9 after HDR. The S92A mutation prevents luteinizing hormone-induced phosphorylation at this site. (J:258770)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pde5a Mutation: 53 strains or lines available

References

• Original: J:258770 Egbert JR, et al., Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis. Dev Biol. 2018 Mar 1;435(1):6-14
• All: 1 reference(s)