Col1a1^{tm6(tetO-Nanog)Hoch}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm6(tetO-Nanog)Hoch}
• Name: collagen, type I, alpha 1; targeted mutation 6, Konrad Hochedlinger
• MGI ID: MGI:6368532
• Synonyms: Col1a1^tetO-Nanog
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm6(tetO-Nanog)Hoch} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279984
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm6(tetO-Nanog)Hoch} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nanog (MGI:1919200)

• Mutation details: A targeting vector was designed to insert an FRT-flanked PGK-Neo-pA 500 bp downstream of the 3' UTR of the Col1a1 locus. The FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus was replaced by co-injection of a tetO-Nanog (Nanog homeobox) plasmid and flpe recombinase. The tetO-Nanog plasmid contained a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of the Nanog gene. (J:279984)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:279984 Palla AR, et al., The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep. 2015 May 19;5:10205
• All: 3 reference(s)