Col1a1^{tm4(tetO-H3f3a*K36M)Hoch}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm4(tetO-H3f3a*K36M)Hoch}
• Name: collagen, type I, alpha 1; targeted mutation 4, Konrad Hochedlinger
• MGI ID: MGI:6368158
• Synonyms: Col1a1-tetO-H3.3K36M
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm4(tetO-H3f3a*K36M)Hoch} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341515
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm4(tetO-H3f3a*K36M)Hoch} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	H3f3a (MGI:1097686)	contains a K36M amino acid substitution

• Col1a1^{tm4(tetO-H3f3a*K36M)Hoch} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: The targeting vector is designed to insert an FRT-flanked PGK-Neo-pA 500 bp downstream of the 3' UTR of the Col1a1 locus. The FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus is then replaced by co-injection of a mutant tetO-H3.3f3a plasmid and flpe recombinase. The plasmid contains a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of the H3.3 histone A gene carrying a lysine-to-methionine substitution at amino acid 36 (K36M). (J:341515)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:341515 Brumbaugh J, et al., Inducible histone K-to-M mutations are dynamic tools to probe the physiological role of site-specific histone methylation in vitro and in vivo. Nat Cell Biol. 2019 Nov;21(11):1449-1461
• All: 2 reference(s)