Rr27^tm2.1Keij
Targeted Allele Detail

Summary

• Symbol: Rr27^tm2.1Keij
• Name: regulatory region 27; targeted mutation 2.1, Keiji Tanimoto
• MGI ID: MGI:6368142
• Synonyms: ICR^Insulated
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm2.1Keij page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226694
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutation: Insertion
• Mutation details: A pair of 237 bp chicken beta globin HS4 insulator core fragments cHS4c was inserted dowstream of the H19 ICR and a second pair, along with a loxP site flanked neomycin resistance gene cassette, upstream. Cre-mediated recombination removed the neo cassette. (J:226694)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:226694 Matsuzaki H, et al., De novo DNA methylation through the 5'-segment of the H19 ICR maintains its imprint during early embryogenesis. Development. 2015 Nov 15;142(22):3833-44
• All: 1 reference(s)