Ep300^tm1.1Few
Targeted Allele Detail

Summary

• Symbol: Ep300^tm1.1Few
• Name: E1A binding protein p300; targeted mutation 1.1, Fredric E Wondisford
• MGI ID: MGI:6364137
• Synonyms: p300(G422S), p300G422S-KI, p300KI
• Gene: Ep300 Location: Chr15:81470329-81536273 bp, + strand Genetic Position: Chr15, 38.16 cM
• Alliance: Ep300^tm1.1Few page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278697
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Nucleotide substitutions
• Mutation details: A mutation was engineered to change glycine codon 422 to a serine codon (p.G422S). This change renders the peptide subject to phosphorylation at that residue and thus makes it more similar to homolog Crebbp, which has a serine at the equivalent position (S436). A self-excising ACN cassette (comprising a loxP site, testes-specific elements of mouse Ace gene, a modified Cre gene with SV40 intron and HSV polyadenylation signal, a neomycin resistance gene cassette, and a second loxP site) was inserted into intron 5. (J:278697)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ep300 Mutation: 98 strains or lines available

References

• Original: J:278697 He L, et al., Transcriptional co-activator p300 maintains basal hepatic gluconeogenesis. J Biol Chem. 2012 Sep 14;287(38):32069-77
• All: 3 reference(s)