Igs2^{tm1(CAG-dcas9/KRAB)Kajim}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(CAG-dcas9/KRAB)Kajim}
• Name: intergenic site 2; targeted mutation 1, Shingo Kajimura
• MGI ID: MGI:6361664
• Synonyms: dCas9-KRAB, Igs2^{tm1(CAG-cas9*)Kajim}
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(CAG-dcas9/KRAB)Kajim} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278806
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: STOCK Igs2^tm3.1Luo/Luo

Mutation description

• Allele Type: Targeted (Endonuclease, Reporter)
• Mutation: Insertion
• Mutation details: A site-specific integrase-mediated approach inserted attP site, attL site, CAG promoter, kinase dead cas9 gene, SV40 polyadenylation sequence and attR site. The targeting construct also contained an mCherry reporter gene and puromycin resistance cassette. (J:278806)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying the Igs2^tm3.1Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 ES cells on a mixed background with 129S1/SvJ, 129X1/SvJ, C57BL/6J, DBA/2J, and FVB/N.

References

• Original: J:278806 Yoneshiro T, et al., BCAA catabolism in brown fat controls energy homeostasis through SLC25A44. Nature. 2019 Aug;572(7771):614-619
• All: 3 reference(s)