Sftpc^tm1Mfbs
Targeted Allele Detail

Summary

• Symbol: Sftpc^tm1Mfbs
• Name: surfactant associated protein C; targeted mutation 1, Michael F Beers
• MGI ID: MGI:6360467
• Synonyms: Sftpc^I73T-neo
• Gene: Sftpc Location: Chr14:70758389-70761521 bp, - strand Genetic Position: Chr14, 36.32 cM
• Alliance: Sftpc^tm1Mfbs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267027
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Epitope tag)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Sequence coding for an N-terminal hemagglutinin (HA) tag was inserted into exon 1 immediately after the ATG start codon, isoleucine codon 73 (ATC) in exon 3 was changed to threonine (p.I73T), and an FRT site flanked neomycin resistance gene and gb2-promoter-driven kanamycin resistance gene cassette into intron 1. The neo/kana cassette interferes with the expression of the gene and mRNA expression is at 15%. (J:267027)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sftpc Mutation: 22 strains or lines available

References

• Original: J:267027 Nureki SI, et al., Expression of mutant Sftpc in murine alveolar epithelia drives spontaneous lung fibrosis. J Clin Invest. 2018 Aug 31;128(9):4008-4024
• All: 2 reference(s)