Mettl4^em1Dfel
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mettl4^em1Dfel
• Name: methyltransferase 4, N6-adenosine; endonuclease-mediated mutation 1, Douglas E Feldman
• MGI ID: MGI:6358564
• Synonyms: Mettl4^em6Dfel
• Gene: Mettl4 Location: Chr17:95030018-95057447 bp, - strand Genetic Position: Chr17, 61.17 cM
• Alliance: Mettl4^em1Dfel page

Mutation origin

• Strain of Origin: (C57BL/6 x 129)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: Cas9 mRNA and single-guide RNA (sgRNA) targeting murine Mettl4 exon 6 was microinjected into the cytoplasm of zygote-stage embryos to induce deletions. Injected embryos were implanted in CD-1 foster mice and pups screened for deletions. From a single round of microinjection, five mice were born and four contained deletions of exon 6. Three founder mice were selected for mouse line expansion prior to experimental analysis. This allele has deletion of exon 6, which creates a translational frameshift that ablates the catalytic domain, for a predicted methyltransferase knockout (KO) allele. (J:278437)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mettl4 Mutation: 28 strains or lines available

References

• Original: J:278437 Kweon SM, et al., An Adversarial DNA N(6)-Methyladenine-Sensor Network Preserves Polycomb Silencing. Mol Cell. 2019 Jun 20;74(6):1138-1147.e6
• All: 1 reference(s)