Fzd4^em1Nat
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fzd4^em1Nat
• Name: frizzled class receptor 4; endonuclease-mediated mutation 1, Jeremy Nathans
• MGI ID: MGI:6358194
• Synonyms: Fz4^MGK
• Gene: Fzd4 Location: Chr7:89053574-89062341 bp, + strand Genetic Position: Chr7, 49.32 cM
• Alliance: Fzd4^em1Nat page

Mutation origin

• Strain of Origin: (C57BL/6 x SJL)F2

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 genome editing is used to modify amino acids TTV (nucleotides ACTGTGGTA) to MGK (nucleotides ATGGGCAAG) in exon 2. As part of the repair of the CRISPR cleavage, there is a 40 bp insert immediately 3' of the stop codon, which is useful for PCR genotyping. (J:275974)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fzd4 Mutation: 33 strains or lines available

References

• Original: J:275974 Cho C, et al., Dlg1 activates beta-catenin signaling to regulate retinal angiogenesis and the blood-retina and blood-brain barriers. Elife. 2019 May 8;8:e45542
• All: 1 reference(s)