Magi1^tm1Itl
Targeted Allele Detail

Summary

• Symbol: Magi1^tm1Itl
• Name: membrane associated guanylate kinase, WW and PDZ domain containing 1; targeted mutation 1, Ingenious Targeting Laboratory
• MGI ID: MGI:6356800
• Gene: Magi1 Location: Chr6:93652436-94260898 bp, - strand Genetic Position: Chr6, 42.7 cM, cytoband D3
• Alliance: Magi1^tm1Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237632
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Not Specified
• Mutation details: A neo cassette replaced 3.2 kb of Mag1 genomic DNA, including most of exon 1 together with its ATG start codon as well as its upstream promoter region. This is a null allele of all three splice isoforms. Western blotting of the kidney cortex confirmed complete loss of Magi1 protein expression in the null mice. (J:237632)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Magi1 Mutation: 174 strains or lines available

References

• Original: J:237632 Ni J, et al., MAGI-1 Interacts with Nephrin to Maintain Slit Diaphragm Structure through Enhanced Rap1 Activation in Podocytes. J Biol Chem. 2016 Nov 18;291(47):24406-24417
• All: 1 reference(s)