Stk38l^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Stk38l^{tm1b(KOMP)Wtsi}
• Name: serine/threonine kinase 38 like; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6356401
• Gene: Stk38l Location: Chr6:146626493-146680310 bp, + strand Genetic Position: Chr6, 77.7 cM
• Alliance: Stk38l^{tm1b(KOMP)Wtsi} page
• IMPC: Stk38l gene page

Mutation origin

• Mutant Cell Line: EPD0396_5_A05
• Germline Transmission: Earliest citation of germline transmission: J:265036
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 146669464 of Chromosome 6 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 146670075. The critical exon 7 is thus flanked by loxP sites. The lacZ open reading frame is not in frame with exon 6 and not expected to produce a reporter protein (confirmed by immunoblotting). Cre expression without flp expression generated a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:265036)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stk38l Mutation: 37 strains or lines available

References

• Original: J:265036 Leger H, et al., Ndr kinases regulate retinal interneuron proliferation and homeostasis. Sci Rep. 2018 Aug 22;8(1):12544
• All: 1 reference(s)