Apmap^tm1.2Jgbs
Targeted Allele Detail

Summary

• Symbol: Apmap^tm1.2Jgbs
• Name: adipocyte plasma membrane associated protein; targeted mutation 1.2, Juliane Bogner-Strauss
• MGI ID: MGI:6343493
• Synonyms: ApmapE1-KO
• Gene: Apmap Location: Chr2:150425000-150450487 bp, - strand Genetic Position: Chr2, 74.68 cM
• Alliance: Apmap^tm1.2Jgbs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:247647
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: Exon 1 was deleted after germline recombination of loxP sites. Western blot analysis confirmed the absence of full-length 46-kDa APMAP protein in all tested tissues of homozygous mutant mice. A second protein band of about 42 kDa was detected in all adipose tissue (AT) depots (epididymal WAT, stromal WAT, mesenteric and perirenal WATs, and brown AT) and was not affected by the deletion of exon 1. Mass spectrometry confirmed that the 42-kDa band detected by immunoblot analysis is a truncated version of APMAP protein. The mouse AT-specific APMAP isoform is independently transcribed from exon 2, as verified by 5' RACE analysis. The knock-out mouse model described in J:247647 lacks the full-length APMAP variant, i.e. the only isoform expressed in humans. (J:247647)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apmap Mutation: 19 strains or lines available

References

• Original: J:247647 Pessentheiner AR, et al., APMAP interacts with lysyl oxidase-like proteins, and disruption of Apmap leads to beneficial visceral adipose tissue expansion. FASEB J. 2017 Sep;31(9):4088-4103
• All: 1 reference(s)