Naa10^tm1.1Yzhg
Targeted Allele Detail

Summary

• Symbol: Naa10^tm1.1Yzhg
• Name: N(alpha)-acetyltransferase 10, NatA catalytic subunit; targeted mutation 1.1, Yi Zhang
• MGI ID: MGI:6315272
• Synonyms: Naa10^-
• Gene: Naa10 Location: ChrX:72960479-72965550 bp, - strand Genetic Position: ChrX, 37.49 cM
• Alliance: Naa10^tm1.1Yzhg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251884
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 1 and an frt-flanked neomycin selection cassette followed by second loxP site were inserted into intron 6. Exons 2 to 6 and the neo cassette were thus flanked by loxP sites. Cre mediated recombination resulted in the deletion of exons 2-6 and the neomycin selection cassette. Western blot analysis demonstrated that the encoded protein is undetectable in mouse embryonic fibroblasts derived from hemizygous male mice. (J:251884)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Naa10 Mutation: 10 strains or lines available

References

• Original: J:251884 Lee CC, et al., The Role of N-alpha-acetyltransferase 10 Protein in DNA Methylation and Genomic Imprinting. Mol Cell. 2017 Oct 5;68(1):89-103.e7
• All: 1 reference(s)