Col1a1^{tm1.1(tetO-Gfi1,-Venus)Hlg}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1.1(tetO-Gfi1,-Venus)Hlg}
• Name: collagen, type I, alpha 1; targeted mutation 1.1, H Leighton Grimes
• MGI ID: MGI:6281947
• Synonyms: G3GV, Gfi1-IRES-Venus
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1.1(tetO-Gfi1,-Venus)Hlg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271545
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1.1(tetO-Gfi1,-Venus)Hlg} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gfi1 (MGI:103170)

• Col1a1^{tm1.1(tetO-Gfi1,-Venus)Hlg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: The targeting vector contains sequence encoding the mouse Gfi1 open reading frame, followed by an IRES-Venus (YFP variant) under the control of the tetO (tetracycline responsive element). The Gfi1-IRES-Venus vector is inserted into the 3' UTR of the Col1a1 locus using a FLP recombinase expression vector (pCAGs-FLPe-Puro), which also repairs a defective hygromycin resistance gene. (J:271545)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 161 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae}.

References

• Original: J:271545 Olsson A, et al., Single-cell analysis of mixed-lineage states leading to a binary cell fate choice. Nature. 2016 Sep 29;537(7622):698-702
• All: 1 reference(s)