Kras^em1Ldow
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kras^em1Ldow
• Name: Kras proto-oncogene, GTPase; endonuclease-mediated mutation 1, Lukas Dow
• MGI ID: MGI:6281558
• Synonyms: KRAS^G12C, Kras^LSL-G12C
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^em1Ldow page

Mutation origin

• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: CRISPR/Cas9 genome editing is used to alter embryonic stem (ES) cells from Kras^tm4Tyj mutant mice. The original Kras^LSL-G12D mice have a G12D point mutation in exon 2 of the gene and a loxP site flanked STOP element in intron 1. Guide RNAs targeting the Kras^G12D site, an ssODN template and the Cas9 nuclease are used to introduce a p.G12C mutation (c.34_36delGGTinsTGC), commonly seen in non-small cell lung cancer. (J:299292)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 69 strains or lines available

Notes

The ES cell line used to create Kras^tm4Tyj is J1 (129S4/SvJae). The ES cell line also included a pancreas-specific Cre ( Ptf1a^{tm1.1(cre)Cvw}) and a far-red fluorescent Cre reporter ( Gt(ROSA)26Sor^{tm2(CAG-rtTA3,-mKate2)Slowe}).

References

• Original: J:299292 Zafra MP, et al., An In Vivo Kras Allelic Series Reveals Distinct Phenotypes of Common Oncogenic Variants. Cancer Discov. 2020 Nov;10(11):1654-1671
• All: 6 reference(s)