Piezo1^tm2.2Apat
Targeted Allele Detail

Summary

• Symbol: Piezo1^tm2.2Apat
• Name: piezo-type mechanosensitive ion channel component 1; targeted mutation 2.2, Ardem Patapoutian
• MGI ID: MGI:6276240
• Gene: Piezo1 Location: Chr8:123208437-123278068 bp, - strand Genetic Position: Chr8, 71.31 cM
• Alliance: Piezo1^tm2.2Apat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269498
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. The FRT-lacZ-loxP-neo-FRT-loxP cassette is introduced into intron 19 followed by a loxP site placed in intron 23. Flp-and Cre-mediated recombination excised the lacZ and neomycin cassettes and exons 20-23. (J:229436, J:269498)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Piezo1 Mutation: 88 strains or lines available

References

• Original: J:269498 Nonomura K, et al., Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation. Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):12817-12822
• All: 2 reference(s)