Dnd1^em1Jil
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnd1^em1Jil
• Name: DND microRNA-mediated repression inhibitor 1; endonuclease-mediated mutation 1, Jinsong Li
• MGI ID: MGI:6275110
• Synonyms: DND1^E59K
• Gene: Dnd1 Location: Chr18:36896724-36899267 bp, - strand Genetic Position: Chr18, 19.46 cM
• Alliance: Dnd1^em1Jil page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Single point mutation
• Mutation details: The gene was targeted for single base substitutions using a third generation base editor (BE3) that is a fusion protein consisting of rat cytidine deaminase Apobec1, cas9 nickase (nCas9) and bacteriophage PBS1 uracil glycosylase inhibitor (UGI). The BE3 mRNA was micro-injected into zygotes together with an sgRNA targeting the sequence of one for the desired domains (RRM1, RRM2, DSRM). This allele has a substitution changing glutamic acid codon 59 to lysine (p.E59K). (J:269609)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnd1 Mutation: 16 strains or lines available

References

• Original: J:269609 Li Q, et al., CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Nov;20(11):1315-1325
• All: 1 reference(s)